Some 10X v3 libraries were accidentally sequenced using 26bp barcode instead of 28bp barcode typically used for 10X v3 libraries.

For these libraries,  although the libraries were prepared using 12bp UMI, only the first 10bp UMI were sequenced. These libraries were later re-sequenced with higher sequencing depth, and the re-sequenced libraries were merged for post-processing. Therefore, the fastq files for these re-sequenced libraries contain reads with both 10bp and 12bp UMIs. A small number of UMI molecules were double counted, with a 10bp version and a 12bp version.

Prep method	Sorting Strategy	10X library	Sequencing batch
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_A01	SM-HH8FP-3
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_A01	SM-HH8FO-8
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_B01	SM-HH8FP-4
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_B01	SM-HH8FO-9
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_C01	SM-HH8FP-5
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_C01	SM-HH8FO-10
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_D01	SM-HH8FP-6
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_D01	SM-HH8FO-11
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_E01	SM-HH8FP-7
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_E01	SM-HH8FO-12
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_F01	SM-HPZA3-2
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_F01	SM-HH8FO-13
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_G12	SM-HH8FP-1
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_G12	SM-HH8FO-6
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_H12	SM-HH8FP-2
MOp_v3	Cells_FACS-tdT	L8TX_181211_01_H12	SM-HH8FO-7