Some 10X v3 libraries were accidentally sequenced using 26bp barcode instead of 28bp barcode typically used for 10X v3 libraries.

For these libraries,  although the libraries were prepared using 12bp UMI, only the first 10bp UMI were sequenced. These libraries were later re-sequenced with higher sequencing depth, and the re-sequenced libraries were merged for post-processing. Therefore, the fastq files for these re-sequenced libraries contain reads with both 10bp and 12bp UMIs. A small number of UMI molecules were double counted, with a 10bp version and a 12bp version.

Prep method	Sorting Strategy	10X library	Sequencing batch
MOp_nuclei-v3	Nuclei_FACS-NeuN	L8TX_181211_01_A02	SM-HH8FP-8
MOp_nuclei-v3	Nuclei_FACS-NeuN	L8TX_181211_01_B02	SM-HH8FP-9
MOp_nuclei-v3	Nuclei_FACS-NeuN	L8TX_181211_01_C02	SM-HH8FP-10
MOp_nuclei-v3	Nuclei_FACS-NeuN	L8TX_181211_01_D02	SM-HH8FP-11
MOp_nuclei-v3	Nuclei_FACS-NeuN	L8TX_181211_01_H01	SM-HPZA3-4